Member institution guidelines for application:
Chameleon is a commercial prototype of the Spotiton robot which provides a novel method for vitrifying samples for cryoEM. MEMC member institutions may apply for access to the system by completing a short application as described below. Successful applicants will be provided with staff assisted access to the chameleon for a half day exploratory study. During this time, grids will be prepared using the chameleon and examined on a screening microscope.
If you would like to apply, please fill out this application
Suggested Sample Requirements
| Acceptable | Recommended | ||
| Concentration | 0.5–5 mg/ml | 2x-4x the plunging concentration | |
| Glycerol | 0–10 % | No glycerol | |
| Detergent | As low as possible | No detergent | |
| Stability | 1 hour at RT | 4+ hours at RT | |
| Volume | 10 µl | 25 + µl |
