You can reset your password by going onto our registration website deon.nybsc.org and clicking ‘Forgot Password?’ You should receive an email shortly after.
Your username should be the first initial of your first name followed by your entire last name. For example, Jane Doe’s username would be jdoe. If you’re still having trouble, please email memc [a] nysbc.org.
You need to be an owner of the project in order to share the data. The owner of the project needs to send an email to memc [a] nysbc.org with the following information: their name and LDAP information, the name and LDAP information of the person they want to share ownership with, and the name of the project. Please cc all pertinent individuals in the email as well (labmate/collaborator, your PI, their PI, etc).
The MEMC area and related sample preparation laboratories are classified as BSL-1 laboratories. All samples must be in compliance with our safety guidelines.
I no longer need my screening microscope/plunge freezing session. Why can’t I cancel my session on PPMS?
PPMS does not allow users to cancel their sessions within 24 hours of the session. If you cannot use the time at the last minute, please email memc [a] nysbc.org that you wish to cancel your session.
Please email memc [a] nysbc.org with the assistance request, pending staff availability, your session will either be confirmed or rescheduled.
Staff are assigned to sessions on the Thursday prior of the week of the booked sessions. If you require assistance, we will assign a staff member to help you. You should receive a confirmation email and be able to see who is assigned to assist you for your session.
It is usually held on the first Tuesday of the month from 9:30am to 2pm. Please email memc [a] nysbc.org for availability.
Screening Microscopes/Plunge Freezers
After-hours – or 24 hour – access, is given on a per instrument basis. This means for any instrument you want to book, you must be deemed independent on it by two separate MEMC staff members. To be independent, you must know how to properly start up, operate and shut down the instrument on your own. MEMC reserves the right to revoke ‘independence’ if we believe it is in the best course of action to prevent damage to instrumentation.
We recommend using the F20 and Hitachi for training and screening if it is your first time using cryo-EM. Users can become fully independent. The F20 is also used for data collection using the direct electron detector camera, DE20.
You may bring a guest to your session as long as they are a registered user at the center. If not, guests must be announced ahead of time by emailing memc [a] nysbc.org and must follow the visitor policy. More information about the guest/visitor policy can be found here.
Yes. First, we would recommend obtaining all the necessary consumables (gold wire and grids). Then, we can schedule a time to assist you with turning standard carbon foil on metal grids to gold foil on metal grids. Please email memc [a] nysbc.org once you have these consumables. For more details on the process, click here for information from one of our workshops with slides and instructions. Lastly, we used gold wire from Ted Pella which you can find here.
Please email memc [at] nysbc.org to schedule a grid drop off appointment. Note, if you are dropping off unclipped grids, they are due at the center no later than Wednesday 4:00pm, the week before the scheduled session. If you are dropping off clipped grids, they are due at the center no later than Friday 4:00pm, the week before your scheduled session. We encourage you to drop off grids well before the cut-off time and we accept grids up to 5 weeks ahead of your session. To open the Krios Grid Drop-off Checklist, click here.
Grids are kept in our saved puck inventory for 2 weeks after a session, after that they are warmed up and discarded.
Any interested user should email memc [a] nysbc.org and their institutional representative an application. They must state that they are applying for rapid access time and submit an application. The application should include: a) Leginon project ID (if you do not have one please create one), b) Leginon IDs of the personnel that will be using the instrumentation, and c) a brief update of the progress of the project including why it is ready for the Krios (please include preliminary cryo data). Also, we prefer users to store their grids on site. If you receive time on another instrument you may always retrieve your grids during business days 10am-4pm. More information on rapid access can be found here.
I have already dropped off grids at MEMC and I would like to swap them out for new grids / add grids. Is this possible?
Yes. The grids can be swapped out for new grids or new grids can be added to the ones already dropped off. If they are unclipped, they must be dropped off by Wednesday at 4 pm the week before your session. If they are clipped, they can be dropped off by Friday at 4pm the week before your session. A new Krios Grid Drop-Off Sheet must accompany the grids.
We use Globus for data collection and that process was covered during new user orientation, but you may refer to their help guide here.
For our Titan Krios GIF/K3 systems the references need a y-flip and there is a file in your references folder that explicitly states the gain reference to use. For other parameters, such as pixel size, Cs, etc… you may find them through the webviewer.
For more information please email memc [at] nysbc.org .
Users will not be required to wear a mask if they are able to provide proof of two vaccinations and a booster. If you have tested positive for COVID-19 10-days prior to coming to NYSBC please email memc [a] nysbc.org before coming on-site for further advising.